The interpretation of laboratory test results requires reference or cutoff values. An improved diagnosis with accurate, reliable laboratory reference ranges saves valuable healthcare resources essential for effective clinical evaluation and monitoring. This benefit provides improved cost containment by eliminating unnecessary testing, treatment changes, and use of other healthcare resources during the episode of care. Based on the above fact, this study was designed to establish reference ranges for hematological parameters in apparently healthy voluntary non-remunerated blood donors. The study was a hospital based cross-sectional experimental study conducted between February and October 2020. Blood samples were taken from 356 healthy adults (18 to 60 years) 211 (59.3%) males and 145 (40.7%) females and routine hematology analysis performed. Patients were assessed as healthy on the basis of a medical history and medical examinations. Venous blood from the antecubital fossa was collected in 2-3mL of K_- ethylenediamine tetra acetic acid (EDTA) for complete blood counts. A total of nineteen hematological parameters were tested in this study and showed significant differences (p<0.001) among males and females with the former showing higher CBC values, except WBC, GRAN# and platelet. More than 10% of the female population presented results that were out of that of the accompanying manual of the hematology analyzer used in the study. The reference range for LYM % for the female was above the upper limit of the manufacturers’ manual reference and for GRAN% in the males was below the lower limit of the accompanying manual of the hematology analyzer. Several differences were also observed when compared to previously established values from Yaounde (Cameroon), most notably in platelets. Our findings for CBC parameters, are in general agreement with previously published data from more limited trials undertaken in other African countries. In spite of the uncontrolled factors influencing hematological values, this study permitted to establish new hematological reference values for use in the North West region of Cameroon. In the absence of previously detailed and more comprehensive investigated hematological reference values for the region we offered to use these results for clinical management of patients from this region and interpretations of laboratory data.